![]() ![]() Microorganisms are the most abundant source of natural antibiotics for clinical application, and have the potential to produce many more secondary metabolites than previously thought, which might be used as drug leads 1– 3. The results demonstrate that VRS-bAHL is a powerful tool for advancing genetic regulation studies and discovering valuable active metabolites in microorganisms. With the guidance of VRS-bAHL, a novel oxazolomycin derivative is discovered to the best of our knowledge. Validation assays prove that VRS-bAHL can be widely used for characterizing gene expression in Streptomyces. violaceum, via its violacein production upon AHL induction. AHL synthase gene ( cviI) of Chromobacterium violaceum as reporter gene is expressed in Gram-positive Streptomyces to synthesize AHL, which is detected with CV026, an AHL deficient mutant of C. Here, we report a visualization reporter system based on Gram-negative bacterial acyl-homoserine lactone quorum-sensing (VRS-bAHL). The existing challenge is to devise sensitive reporter systems for mining valuable antibiotics. Genome sequencing reveals that streptomycetes have the potential to produce many more bioactive compounds that may combat the emerging pathogens. ![]() The crisis of antibiotic resistance has become an impending global problem. ![]()
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